It truly is essential that you don't retail store your HPLC column when it continue to consists of any buffers or ion-pairing brokers. To make certain this, flush five column volume
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-hydroxybenzoic acid (PH) with a nonpolar C18 column subject to a optimum Evaluation time of 6 min. The shaded places characterize areas where by a separation is impossible, While using the unresolved solutes discovered.
The mobile section is buffer, and also the column packing comprises ionic gr
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